Scientist Solutions: Life Science Discussions
 Refer a Friend    Link To Us    Bookmark Us       

      
 » Home » Virology » Retrovirus (HIV) » Lentivirus concentration

Other Topics
11/24/2008 11:00 AM
unsolved qPCR problem-HEL ...
9/5/2008 07:28 AM
Virus active after 7 days ...
9/4/2008 11:27 AM
Did the Romans destroy Eu ...
8/8/2008 01:01 PM
HIV cases unreported! Cr ...
4/25/2008 02:25 PM
Atomic Force Microscopy
3/12/2008 02:11 PM
HIV-inactivation time out ...
2/22/2008 07:08 PM
David Baltimore just cla ...
1/29/2008 12:35 AM
problem with long-term st ...
9/30/2007 02:50 PM
HIV rev
8/1/2007 06:19 AM
Comparing treatments in H ...
6/26/2007 05:23 AM
HIV Variants
5/18/2007 02:54 AM
Innovative Web Source on ...
4/20/2007 02:27 PM
Why HIV is so Prevalent i ...
7/14/2006 10:38 PM
Robust HIV-specific immun ...
4/10/2006 10:58 AM
Are there any labs which ...
1/24/2006 01:33 AM
paper hypothesizing hiv f ...
12/14/2005 06:15 PM
Avian Myeloblastosis Viru ...
1/21/2005 12:26 PM
Norwalk Virus Detection
Subscribet to topic
Add Reply  Add New Topic  Add New Poll
bottom of page RSS Feed 

Topic Feed
 

Lentivirus concentration

 [View Printable]
parvoman

Frog Laureate

See
Similar
Scientists




Group: Member
Posts: 272
Joined: Jul 28, 2005







 Send a personal messsage to parvoman Reply with a quote from this post Go to the top of the page



Does anyone have a good protocol for concentrating recombinant VSV-G pseudotyped lenti particles?

Thanks

PM
.........................
 Posted Sep 04, 2007, 23:36 PM
dvatakis

Frog Egg

See
Similar
Scientists




Group: Member
Posts: 6
Joined: Feb 04, 2005







 Send a personal messsage to dvatakis Reply with a quote from this post Go to the top of the page

There are two ways. One is ultracentrifugation which results in loss of titers but allows you to concentrate your virus by factor of 100. On the other hand, there are the Amicon Ultra-15 columns by Milipore that I have used andthey work great. They concentrate your virus prep by at least 30-fold.

.........................
Posted Sep 29, 2007, 9:47 AM
Nephron

Frog Egg

See
Similar
Scientists




Group: Member
Posts: 1
Joined: Jun 25, 2008







 Send a personal messsage to Nephron Reply with a quote from this post Go to the top of the page

dvatakis said:
There are two ways. One is ultracentrifugation which results in loss of titers but allows you to concentrate your virus by factor of 100. On the other hand, there are the Amicon Ultra-15 columns by Milipore that I have used andthey work great. They concentrate your virus prep by at least 30-fold.



How long do you spin the sup containing virus?

.........................
Posted Jun 25, 2008, 18:52 PM
parvoman

Frog Laureate

See
Similar
Scientists




Group: Member
Posts: 272
Joined: Jul 28, 2005







 Send a personal messsage to parvoman Reply with a quote from this post Go to the top of the page

dvatakis said:
There are two ways. One is ultracentrifugation which results in loss of titers but allows you to concentrate your virus by factor of 100. On the other hand, there are the Amicon Ultra-15 columns by Milipore that I have used andthey work great. They concentrate your virus prep by at least 30-fold.



I have used both of these methods before but found the recovery of virus from the filtration device quite poor and resuspension of virus post-ultra-centrifugation to be difficult and to result in loss of infectivity.

Do you use sucrose in the ultracentrifugation and if so at which concentration and volumes etc?

Thanks
.........................
Posted Jun 26, 2008, 17:35 PM
top of page Add Reply  Add New Topic  Add New Poll

Forum Jump