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 Transient transfection [View Printable]
ranjeet

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Joined: Aug 08, 2007







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HI all,
Any one plz help me in sorting out transfection of jurkat cells, I am using biorad electroporator. I had used like kept cells in serum free media for overnight nad then did transfection in medium have no dextrose and no beta ME. I had tried also in trehalose and DMSO containing media. Plz try to tell me good protocol. Can be make expression level high in jurkar cells.
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 Posted Aug 09, 2007, 5:07 AM
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