|Vera Barbosa said:|
I was wondering if someone could answer me this:
How can I measure cytokines using ELISA in a solution that has very small cytokines concentration and high volume of solvent?
I guess you can concentrate your samples using some commercial columns
and check the concentration
and then calculate the real concentration
according to the volumn left.
Volumn A (known)XConcentration A(unknown)=Volumn B (known after concentration
)XConcentration B (from ELISA