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ELISA and low cytokine concentrations

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Vera Barbosa
Portugal

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Topic Started by Vera Barbosa
on 8/4/2007 10:49 AM   
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Hi!
I was wondering if someone could answer me this:
How can I measure cytokines using ELISA in a solution that has very small cytokines concentration and high volume of solvent?


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COHscientist
United States

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Posted By COHscientist
on 8/5/2007 3:26 AM   
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Vera Barbosa said:
Hi!
I was wondering if someone could answer me this:
How can I measure cytokines using ELISA in a solution that has very small cytokines concentration and high volume of solvent?


I guess you can concentrate your samples using some commercial columns and check the concentration of cytokines and then calculate the real concentration according to the volumn left.

Volumn A (known)XConcentration A(unknown)=Volumn B (known after concentration)XConcentration B (from ELISA)



Vera Barbosa
Portugal

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Posted By Vera Barbosa
on 8/5/2007 12:41 PM   
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Hi.
Thank you very much for the idea...

Hope to have enought cytokines concentration to be detected by the ELISA...

I am thinking on analysing the exhalated breath condensate and this fluid has a very small number of molecules and high concentrations of water.

Bye




Jen_Floyd
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Posted By Jen_Floyd
on 12/2/2008 11:10 AM   
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If price is not an issue, Mesoscale has an assay that measures cytokines down to pg/ml. Also cytometric bead assay by BD might be more sensitive than ELISA but it's also pretty pricey.



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