| Vera Barbosa said: |
Hi!
I was wondering if someone could answer me this:
How can I measure cytokines using ELISA in a solution that has very small cytokines concentration and high volume of solvent?
|
I guess you can concentrate your samples using some commercial
columns and check the
concentration of
cytokines and then calculate the real
concentration according to the volumn left.
Volumn A (known)XConcentration A(unknown)=Volumn B (known after
concentration)XConcentration B (from
ELISA)