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ELISA and low cytokine concentrations

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Vera Barbosa

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 Send a personal messsage to Vera Barbosa Reply with a quote from this post Go to the top of the page

Hi!
I was wondering if someone could answer me this:
How can I measure cytokines using ELISA in a solution that has very small cytokines concentration and high volume of solvent?

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 Posted Aug 04, 2007, 12:49 PM
COHscientist

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Vera Barbosa said:
Hi!
I was wondering if someone could answer me this:
How can I measure cytokines using ELISA in a solution that has very small cytokines concentration and high volume of solvent?


I guess you can concentrate your samples using some commercial columns and check the concentration of cytokines and then calculate the real concentration according to the volumn left.

Volumn A (known)XConcentration A(unknown)=Volumn B (known after concentration)XConcentration B (from ELISA)

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Posted Aug 05, 2007, 5:26 AM
Vera Barbosa

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 Send a personal messsage to Vera Barbosa Reply with a quote from this post Go to the top of the page

Hi.
Thank you very much for the idea...

Hope to have enought cytokines concentration to be detected by the ELISA...

I am thinking on analysing the exhalated breath condensate and this fluid has a very small number of molecules and high concentrations of water.

Bye


.........................

Posted Aug 05, 2007, 14:41 PM
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