T cell proliferation with Macrophages |
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Dear all,
I am trying to perform T cell proliferation assays using macrophages as APCs. It does not seem to work, becuase I used live macrophages. So I decided to use killed APCs as accessory cells. I used mitomycin and it does not seem to work too. Is irradiation the best solution. I am using CFSE stained T cell responders.
Can anyone working with macrophages suggest the best protocol for proliferation assays? Thanks
Aspee
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Replies
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Posted By 前程
on 1/7/2010 3:33 AM
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i donot know whether you resovle the problem .i am also having the same idea with you !
but failure!
Last edited Jan 07, 2010, 5:35 AM by 前程
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