Hello sir. mam,

Well in advance i thank you very much .. i have some doubt regarding SDS page.. after pouring the tank buffer shall we run the gel without loading sample. then turn of the power pack and loading the sample and then we run the process.. it makes any usefulness to us.. or else it makes some problem to the proteins or to gel.

What pH should be maintain in the upper part of the tank buffer and lower part of the tank buffer.. if we change any pH alteration in the both buffer will rise any problem ....

i hope your golden comments...