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warter barth of medium

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guosapphire
China

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Topic Started by guosapphire
on 6/25/2007 14:31 PM
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i wonder whether the cytokines or growth factors in the medium would loss their activity after warter barth at 37 degree? i used to warm up medium before experiment.


thanks!


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samm
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Posted By samm on 6/25/2007 20:42 PM
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Cytokines and growth factors degrade over time, even when you store your medium at 4dC, and deteriorate even faster at 37dC (whether in culture or warming in a water bath). However, there is usually enough of an excess. For really finicky cells that require large amts of FBS or exogenous growth factors, you can always aliquot the amt you need (e.g 30 or 40 ml) and warm just that.
Hope this helps...
-sam





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