warter barth of medium |
|
Would you like to save this topic, event, protocol or job so you can find it again easily?
Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.
Available to members only. Please log in or register for your free account now.
|
|
i wonder whether the cytokines or growth factors in the medium would loss their activity after warter barth at 37 degree? i used to warm up medium before experiment.
thanks!
|
Replies
|
|
|
Posted By samm
on 6/25/2007 20:42 PM
|
|
Cytokines and growth factors degrade over time, even when you store your medium at 4dC, and deteriorate even faster at 37dC (whether in culture or warming in a water bath). However, there is usually enough of an excess. For really finicky cells that require large amts of FBS or exogenous growth factors, you can always aliquot the amt you need (e.g 30 or 40 ml) and warm just that.
Hope this helps...
-sam
|
|
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.
By participating in the voting, you will be helping to identify the best topics & replies on the board.
You may vote once for any one post, and you may not vote for your own posts.
A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.
learn more about member points.
|
Become a member & join our
community (It's easy & free)
Already a member? Please log in
|
AbD Serotec
|
|
Worthington Biochemical
|
|
Worthington Biochemical
|
|
8/18/2010
|
|
8/18/2010
|
|
8/18/2010
|
|
8/30/2010
|
|
8/13/2010
|
|
8/6/2010
|
|
|