can anyone please help me understand how to compute protein concentration through quantitative elisa? Im having difficulty calculating the protein concentration on my samples. thank you very much.

Just i have a document that relate to your needs (attachment).
Also could you help me to show the meaning of N/A, N/A2, a...when we use standard curve to calculate the well concentration? How we decide a result in those cases?

Does that mean you have really low concentration in your sample wells and those cannot be determined. I guess you have to lower your dilution of your samples. If your sample is not diluted, that means they are beyond the range of detection.

COHscientist said:

Does that mean you have really low concentration in your sample wells and those cannot be determined. I guess you have to lower your dilution of your samples. If your sample is not diluted, that means they are beyond the range of detection.

Thanks. I actually think so, the results seem out of range. But can we setup the program to show the extrapolation results in those cases? I'm using Data Manager V5.2 to remote the machine.

Hi!

But inst there any other way of analysing low concentrations of cytokines, even if they are below range of the comercial Kits?

Vera Barbosa said:

Hi! But inst there any other way of analysing low concentrations of cytokines, even if they are below range of the comercial Kits?

I guess you can concentrate your samples using some commercial columns and check the concentration of cytokines and then calculate the real concentration according to the volumn left.

Volumn A (known)XConcentration A(unknown)=Volumn B (known after concentration)XConcentration B (from ELISA)