Scientist Solutions: Life Science Discussions
 Refer a Friend    Link To Us    Bookmark Us       

      
 » Home » Immunology » Assay Development & Protocols » Help with CD90/Thy-1 immunostaining

Other Topics
12/3/2008 04:08 AM
ELISA Hook effect
11/20/2008 01:13 PM
T cell isolation from hum ...
11/13/2008 06:32 PM
thioglycollate excretion- ...
11/5/2008 06:56 PM
flow cytometry- mouse spl ...
11/5/2008 02:43 PM
ELISA assay variability
10/30/2008 12:23 PM
MEASURING APOPTOSIS AND N ...
10/29/2008 12:11 PM
weak signal flowcytomtery
10/9/2008 02:00 PM
Simultaneous measurement ...
10/7/2008 03:13 PM
fluorescent cytotoxicity ...
9/8/2008 05:19 PM
Two colour acquisition pr ...
8/25/2008 01:21 PM
Permeabilization of cells ...
8/24/2008 08:16 PM
hyaluronidase assay
8/14/2008 05:15 PM
Buffers
8/5/2008 01:27 AM
DC generation with Bone M ...
7/30/2008 01:30 PM
Help with CFSE staining o ...
7/25/2008 10:09 AM
desperately in need of he ...
7/24/2008 03:39 AM
A Good Chinese Immunology ...
7/23/2008 12:59 PM
Where to buy mouse PBMC a ...
6/26/2008 05:58 PM
Flow Scatter Plots
6/19/2008 11:50 PM
T cell isolation from hum ...
5/21/2008 12:27 AM
lipids ultracentrifugatio ...
5/8/2008 10:26 AM
MTT assay
4/5/2008 04:17 PM
Four parameter standard c ...
1/26/2008 05:46 AM
Fixation and 7-AAD
1/22/2008 08:41 AM
Isostype Controls in Flow
11/4/2007 02:31 AM
Chicken Immunoglobulin Pu ...
11/2/2007 12:26 PM
Isolation of mouse T cell ...
10/28/2007 09:46 PM
Blocking surface receptor ...
10/12/2007 06:07 PM
mouse spleen cells
10/3/2007 06:21 AM
help with sodium thioglyc ...
Subscribet to topic
Add Reply  Add New Topic  Add New Poll
bottom of page RSS Feed 

Topic Feed

 

Help with CD90/Thy-1 immunostaining

 [View Printable]
mtakkenberg

Frog Egg

See
Similar
Scientists





Group: Member
Posts: 1
Joined: May 25, 2007







 Send a personal messsage to mtakkenberg Reply with a quote from this post Go to the top of the page

Has anyone ever done immunostaining of paraffin embedded sections with CD90/Thy-1 antibodies? I'm trying to get a working protocol, but I'm having a very hard time....
Can anyone help me out?

.........................

 Posted May 25, 2007, 13:11 PM
samm

Frog Laureate

See
Similar
Scientists



View Blogs


Group: Moderators
Posts: 407
Joined: Mar 03, 2005







 Send a personal messsage to samm Reply with a quote from this post Go to the top of the page

You need to ensure you do the antigen retreival/exposure step (pressure cooking or microwaving) and queching before you stain. I have done this by pressure cooking in a citrate buffer.

.........................

Posted Aug 01, 2007, 19:24 PM
top of page Add Reply  Add New Topic  Add New Poll

Forum Jump