I send my pure samples that inserted in PJET cloning kit .to be sequenced in a company and select the primer M 13(-12) ,But sequencing is failed. why?

sezzat:

Would you please provide some additional information, so that we may help you.

How exactly did your sequencing fail? What did the sequencing company report back to you? Which sequencer and techniques were they using for sequencing?

they told me that sequencing is failed and gave me a sequencing summary with very strang repetations and with different sequence positions , in one sample 113-163of sequence and in another 392-411 of sequence and so on.
I am not sure they use sanger sequencing on ABI machine or GS20/FLx system .
Again ,All my samples (DOPR1-OAR2 and OAMB)are inserted in PJET .

Are you sure the sequencing company used the right primer for sequencing?