Hi all,

Does anyone have a good quantitative RT-PCR protocol for estimating miRNA expression in cells ? I do not want to go back to Northern blots thats all.

Ambion is a great resource for all things miRNA. Here are some useful links for miRNA expression using quantitative RT-PCR.


Ambion Technote 12(4) for mirVana qRT-PCR miRNA Detection Kit and Primer Sets
Quantify MicroRNA Expression by RT-PCR


Ambion Experimental Workflow
miRNA Expression Analysis


Ambion Technote 13(4)
Getting Started with microRNA Expression Analysis Research using Real-Time PCR


Ambion's miRNA Database Search


Ambion Technical Bulletin #508
miRNA (microRNA) Reference List


Ambion
miRNA Research Guide - pdf document




Here are a couple of good references for miRNA expression using qRT-PCR:

Diana O Perkins, Clark D Jeffries, L Fredrik Jarskog, J
Michael Thomson, KeithWoods, Martin A Newman, Joel S Parker,
Jianping Jin and Scott M Hammond. microRNA expression in the prefrontal cortex of individuals with schizophrenia and schizoaffective disorder. Genome Biology 2007, 8:R27


Shingara J, Keiger K, Shelton J, Laosinchai-Wolf W, Powers P, Conrad R, Brown D, Labourier E. An optimized isolation and labeling platform for accurate microRNA expression profiling. RNA. 2005 Sep;11(9):1461-70. Epub 2005 Jul 25.