Hi all,
Dose any one have an expireance with triple staining, using three different sizes of gold particles?

I don't have a lot of experience myself, but it looks like there is a good reference (listed below). They describe using 3 sizes of gold that decorate membranes and are clearly distinguishable. Their sizes for gold were 5, 20 and 40nm.


Journal The Histochemical Journal

Volume 21, Number 3 / March, 1989/136-144

Good luck!

Thanks for the information
Guy