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scFv

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ding

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 Send a personal messsage to ding Reply with a quote from this post Go to the top of the page

In which order are VL and VH more likely to give a good scFv, VH-VL or VL-VH (N- to C-terminus)?

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Posted May 01, 2007, 20:41 PM
samm

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 Send a personal messsage to samm Reply with a quote from this post Go to the top of the page

There was an insert for the Immunoogy "Gold Book" (Current protocols...) that reviews the various options there are - you might want to take a look at that if you haven't done so already.
Also, I've seen similar articles in journals such as Mol. Biotechnol. and Mol. Immunol. Also, companies like FusionAntibodies and GenwayBio might be useful.

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Posted Oct 25, 2007, 14:28 PM
Shubhangi

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Hi would like to share my experience.
For no apparent reasons we didn't find VL-VH working. But Vh-VL worked. We found expression, proper folding.
But the reasons i am not sure.

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Posted Dec 03, 2008, 4:11 AM
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