| satty said: |
| thank you guy for yr timely help.What abt the answer to my second question? why are there so many bands in sds and not in native page? |
in SDS gel, your proteins are denatured, and all proteins appeared to be bands
well, in a native gel, your proteins are still in their native format, i.e., they are still in the protein complex they belong,
so, to your question, a native gel separates native protein complexes, and SDS gel separates individual proteins.
let's say that if you have ten proteins in a complex, which will be separated on a native gel as one band, but will be 10 on a sds gel
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