i'm a BA student from denmark and i'm trying to synthesize a ibuprofen-glucose-esther with a lipase from rhizomucor miehei as a catalyst. in order to analyze my product i need to stop the reaction - preferably without heating.

does anyone know how to denaturate the enzyme without heating the reaction?

stud.polyt.bio said:

i'm a BA student from denmark and i'm trying to synthesize a ibuprofen-glucose-esther with a lipase from rhizomucor miehei as a catalyst. in order to analyze my product i need to stop the reaction - preferably without heating. does anyone know how to denaturate the enzyme without heating the reaction?

Im unsure about the lipase you are using, but many lipases do not function at high pH. Should be easy to search for that for your liapse. For instance, lipoprotein lipase is inhibited above ph 8.5.

Let us know what you figure out

Rb

i'd prefer NOT to manipulate with temperature or pH since it may interfere with my other reagents. what i'm looking for is some kind of chemical denaturation - if possible...

stud.polyt.bio said:

i'm a BA student from denmark and i'm trying to synthesize a ibuprofen-glucose-esther with a lipase from rhizomucor miehei as a catalyst. in order to analyze my product i need to stop the reaction - preferably without heating. does anyone know how to denaturate the enzyme without heating the reaction?

For your solutions, would it work to partition it away with several non-aqueous extractions? Lipases are highly partitioned at lipid interfaces.