Hello everybody,
I need pBudCE4.1 plasmid for my project. Is there anyone who has it?
Burcu Sengez
Izmir Institute of Technology
Biotechnology and Bioengineering Department (MS)
Izmir, Turkey

bsengez:

I'm not sure what you fundings situation is but if you have the resources you can purchase this plasmid from Invitrogen.

Here is their description of this plasmid as well as a link to their page:

Express Two Proteins from a Single Vector

Description:
The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in the cell. This can eliminate variable expression due to differences in gene copy number. pBudCE4.1 provides expression cassettes withthe following features:

* The CMV promoter for high-level transcription of genes with
an optional c-myc epitope tag for rapid detection and 6xHis
sequence for simple purification

* The human EF-1 promoter for high-level expression of
genes with an optional V5 epitope tag for rapid detection
and 6xHis sequence for simple purification

* The Sh ble (ZeoR) gene for efficient selection in both
mammalian cells and E. coli with the selection agent
Zeocin

Contents and Storage:

20 g each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive
control vectors are supplied supercoiled and lyophilized.
Store at -20°C. Vectors are guaranteed stable for 6 months
when properly stored.

Weblink:
pBudCE4.1 (Invitrogen Catalog Number - V532-20)

Hello Mr. Rook,
thanks for your attention. I got the information from Invitrogen page before deciding to use it.
I'm a master student,but unfortunately I cannot afford it. Because of the fact that, I'm looking forward to hearing about someone who has it and can send to me?

To whom sent a private message to me:
I got a message that I have a private message, but unfortunately I couldn't open it. If you are the one who sent it, could you please send it to b_sengez@hotmail.com
Sincerely..

bsengez:

You can view your private messages by mousing over the My Account tab (to the right). Click on the pop-up that say My Messages. This will bring you to a list of your private messages.

Thanks Tony, I know how to look them, but unfortunately I couldn't. Yesterday evening, I was able to reach my messages.
Thanks again for your attention..