bsengez:
I'm not sure what you fundings situation is but if you have the resources you can purchase this plasmid from Invitrogen.
Here is their description of this plasmid as well as a link to their page:
Express Two Proteins from a Single Vector
Description:
The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in the cell. This can eliminate variable expression due to differences in gene copy number. pBudCE4.1 provides expression cassettes withthe following features:
* The CMV promoter for high-level transcription of genes with
an optional c-myc epitope tag for rapid detection and 6xHis
sequence for simple purification
* The human EF-1 promoter for high-level expression of
genes with an optional V5 epitope tag for rapid detection
and 6xHis sequence for simple purification
* The Sh ble (ZeoR) gene for efficient selection in both
mammalian cells and E. coli with the selection agent
Zeocin
Contents and Storage:
20 g each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive
control vectors are supplied supercoiled and lyophilized.
Store at -20°C. Vectors are guaranteed stable for 6 months
when properly stored.
Weblink:
pBudCE4.1 (Invitrogen Catalog Number - V532-20)
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