| Do you want to just knock down your gene of interest? Choose the good-old siRNA style and you should produce a plethora of siRNAs from one construct that 'hopefully' don't have off-target effects. |
An siRNA knockdown
without off-target effects would be an unlikely outcome.
Comparison of siRNA
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Retraction of synapses and dendritic spines induced by off-target effects of RNA interference
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3' UTR seed matches, but not overall identity, are associated with RNAi
off-targets. Birmingham A, Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, Fedorov Y, Baskerville S, Maksimova E, Robinson K, Karpilow J, Marshall WS, Khvorova A. Nat Methods. 2006 Mar;3(3):199-204.
-dependant stimulation and repression of mammalian gene expression
by small interfering RNAs (siRNAs). Persengiev SP, Zhu X and Green M. RNA
Transcriptional gene silencing
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Small interfering RNA
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in human cells
. Morris KV, Chan SWL, Jacobson SE, Looney DJ. Science
A rapid and sensitive assay
efficiency and specificity. Smart N, Scambler PJ, Riley PR. Biol Proced Online. 2005;7:1-7. Epub 2005 Jan 24.
Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins
in mammalian cells
. Scacheri PC, Rozenblatt-Rosen O, Caplen NJ, Wolfsberg TG, Umayam L, Lee JC, Hughes CM, Shanmugam KS, Bhattacharjee A, Meyerson M, Collins FS. Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1892-7. Epub 2004 Feb 09.
Noise amidst the silence: off-target effects of siRNAs? Jackson AL, Linsley PS. Trends Genet. 2004 20(11):521-4
Expression profiling reveals off-target gene
regulation by RNAi
. Jackson AL, Bartz SR, Schelter J, Kobayashi SV, Burchard J, Mao M, Li B, Cavet G, Linsley PS. Nat Biotechnol. 2003 Jun;21(6):635-637.
Small RNAs with Imperfect Match to Endogenous mRNA
: Implications for off-target activity of small inhibitory RNA
in mammalian cells
. Saxena S, Jonsson ZO, Dutta A. J Biol Chem. 2003 Nov 7;278(45):44312-44319.