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Proteasome inhibitor [View Printable]
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gil
Group: Member
Posts: 8
Joined: Oct 17, 2006
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I used the proteasome inhibitor MG132, and the positive control (untreated cells - only MG132) gave me several bands, i know that its a phosphorylation procedure... could anyone explain to me what is happening here?
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| Posted Mar 07, 2007, 21:58 PM |
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gsovak
Group: Member
Posts: 544
Joined: Jan 25, 2005
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Hi There,
I am also using MG132 .
please give me more information about your procedure.
Positive control , you loaded just MG132 after dilution. In what did you diluted it.
if you can attach a pic of the gel will help.
Guy
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| Posted Mar 08, 2007, 19:58 PM |
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