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marijna

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I am looking for information about other electrophoresis condition deferences about standart condition (1v/cm) in comet assay procedure?
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 Posted Feb 27, 2007, 8:16 AM
marijna

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marijna said:
I am looking for information about other electrophoresis condition different other than standart condition (1v/cm) in comet assay procedure?
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Posted Feb 27, 2007, 8:27 AM
Jamie_Cruikshank

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If you are looking for ways to tweak the protocol for the comet assay, check out http://www.cometassay.com/ which explains how this technique identifies and measures DNA breaks, and can be used in the detection of UV induced pyrimidine dimers, oxidized bases, and alkylation damage by using lesion-specific endounucleases.

If you are looking for completely alternative techniques for electrophoresis, BioRad offers equipment for TTGE (Temporal Temperature Gel Electrophoresis) or DGGE gradients to further analyze fragments using variations in temperature, while pushing through the DCode system using adjustable voltage. These boxes have three factors that help seperate your DNA fragments (time, temperature, and voltage), so you can figure the appropriate quantities of each through simple research, that works best for your fragment of choice by evaluating melting temp, and how G-C rich the frag. is.
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Posted Feb 28, 2007, 18:13 PM
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