Hi,

has anybody had used the botulinum toxin A (BoNT/A) to block exocytosys in MCF-7 or epithelial cells (like HeLa)? ... I have been searching an answer on pubmed and could not find anything very satisfaying...

Thank you

Nothing on breast cancer cell lines, but if you search
"BoNT/A SNAP-25 cancer" then you get the following hits



1: Dong M, Tepp WH, Johnson EA, Chapman ER.
Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in
living cells.
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14701-6. Epub 2004 Oct 1.
PMID: 15465919 [PubMed - indexed for MEDLINE]

2: Yowler BC, Kensinger RD, Schengrund CL.
Botulinum neurotoxin A activity is dependent upon the presence of specific
gangliosides in neuroblastoma cells expressing synaptotagmin I.
J Biol Chem. 2002 Sep 6;277(36):32815-9. Epub 2002 Jun 27.
PMID: 12089155 [PubMed - indexed for MEDLINE]

3: Purkiss JR, Friis LM, Doward S, Quinn CP.
Clostridium botulinum neurotoxins act with a wide range of potencies on SH-SY5Y
human neuroblastoma cells.
Neurotoxicology. 2001 Aug;22(4):447-53.
PMID: 11577803 [PubMed - indexed for MEDLINE]

4: Huang X, Kang YH, Pasyk EA, Sheu L, Wheeler MB, Trimble WS, Salapatek A,
Gaisano HY.
Ca(2+) influx and cAMP elevation overcame botulinum toxin A but not tetanus toxin
inhibition of insulin exocytosis.
Am J Physiol Cell Physiol. 2001 Sep;281(3):C740-50.
PMID: 11502551 [PubMed - indexed for MEDLINE]

5: Huang X, Wheeler MB, Kang YH, Sheu L, Lukacs GL, Trimble WS, Gaisano HY.
Truncated SNAP-25 (1-197), like botulinum neurotoxin A, can inhibit insulin
secretion from HIT-T15 insulinoma cells.
Mol Endocrinol. 1998 Jul;12(7):1060-70.
PMID: 9658409 [PubMed - indexed for MEDLINE]