Hi all
Just wanted to let you all know that there is a very interesting article about IR protein detection in Proteomics .Volume 7, Issue 4(No. 4 February 2007)

The name of the article is:
Infrared-based protein detection arrays for
quantitative proteomics
Christian Loebke, Holger Sueltmann, Christian Schmidt, Frauke Henjes,
Stefan Wiemann, Annemarie Poustka and Ulrike Korf
Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany

I copied the abstract:
The advancement of efficient technologies to comply with the needs of systems biology and drug
discovery has so far not received adequate attention. A substantial bottleneck for the timeresolved
quantitative description of signaling networks is the limited throughput and the inadequate
sensitivity of currently established methods. Here, we present an improved protein
microarray-based approach towards the sensitive detection of proteins in the fg-range which is
based on signal detection in the near-infrared range. The high sensitivity of the assay permits the
specific quantification of proteins derived from as little as only 20 000 cells with an error rate of
only 5%. The capacity is limited to the analysis of up to 500 different samples per microarray.
Protein abundance is determined qualitatively, and quantitatively, if recombinant protein is
available. This novel approach was called IPAQ (infrared-based protein arrays with quantitative
readout). IPAQ offers a highly sensitive experimental approach superior to the established
standard protein quantification technologies, and is suitable for quantitative proteomics.
Employing the IPAQ approach, a detailed analysis of activated signaling networks in biopsy
samples and of crosstalk between signaling modules as required in drug discovery strategies can
easily be performed.
Guy