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 Oligofectamine and cell death [View Printable]
gsovak

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Hi All,
I am using Lipofectamine for siRNA transfection of Hela cells.
I have got some problems with cell death, I guess the toxicity of the transfection reagent. ANy one had problems like that before?
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Posted Jan 23, 2007, 17:45 PM
BenchGuy

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I have had seen similar issues with lipid reagents. I use GeneJammer from Stratagene - it works well and it is a lot less toxic.
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Posted Jan 26, 2007, 19:31 PM
gsovak

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Thank For the answer,
And may I welcome you to Scientist solutions.
I will try the product that you mentioned. Can you post a protocol that works for you.
Guy

PS
please fill in your "My BenchSpace", it helps us scientist to create small scientific comunities.
Guy
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Posted Jan 26, 2007, 19:55 PM
montgomj

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I was transfecting siRNA duplexes into some glioma cells and encountered difficulty. After trying several transfection reagents, I found Lipofectamine RNAiMAX to be the best for my cells.

For a quick check of transfection efficiency and cytotoxicity Ambion has a kit for easily assessing GAPDH knockdown. I found it very useful for optimizing my transfection protocol.

http://www.ambion.com/catalog/CatNum.php?AM1639
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Posted Feb 25, 2007, 19:26 PM
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