trook said:

Does anybody have a suggestion for the best imaging methods to be used for Self Assembling Monolayers? I currently have access to light microscope and would like to be able to image a SAM (silane) after attachment to a glass surface. Any suggestions or references?

Please specify the size and the nature of particles you like to see assembeld. Microscopes might have a range of size limit for these type of particles.

trook said:

Does anybody have a suggestion for the best imaging methods to be used for Self Assembling Monolayers? I currently have access to light microscope and would like to be able to image a SAM (silane) after attachment to a glass surface. Any suggestions or references?

I believe the SAM layer is around a few hundred angstroms but no larger than a 1/2 micron. However the SAM may be able to be linked with a marker (fluorescent or visible dye) which may be imaged. Are there any standard methods out there to show that the SAM has actually attached to the surface (imaging or other)?

trook said:

trook said: Does anybody have a suggestion for the best imaging methods to be used for Self Assembling Monolayers? I currently have access to light microscope and would like to be able to image a SAM (silane) after attachment to a glass surface. Any suggestions or references? I believe the SAM layer is around a few hundred angstroms but no larger than a 1/2 micron. However the SAM may be able to be linked with a marker (fluorescent or visible dye) which may be imaged. Are there any standard methods out there to show that the SAM has actually attached to the surface (imaging or other)?

Contact NanoSight Ltd. at www.nanosight.co.uk or send email to: bobcarr@nanosight.co.uk may be they can help to answer your questions. Check big companies such as:
Leica: www.leica-microsystems.com or Olympus: www.microscopy-analysis.com/readers/enquiry.htm