Can anyone please guide me on a good protocol for washing of Inclusion bodies to free them of membrane contaminant and without causing any loss of the desired protein in inclusion bodies. our protein is a cytokine expressed in inclusion bodies by thermal induction.

kindly help me on this.

Here are a couple of protocols for washing of inclusion bodies.

This protocol describes isolation and washing of the inclusion bodies. These protocols are adapted from those originally described by David Garboczi.
http://www.microbiology.emory.edu/altman/jdaWebSite_v3/p_tetIBprep.shtml


Purification of Expressed Proteins from Inclusion Bodies
http://www.biosupplynet.com/cfdocs/protocols/protocol-oct-2006-2.cfm

Hope these help.

These two protocols can be found in our protocol section under protein chemistry.

Direct links:

http://www.scientistsolutions.com/index.php?a=protocol&c=30&p=640

http://www.scientistsolutions.com/index.php?a=protocol&c=30&p=641