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western blotting [View Printable]
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bugme
Group: Member
Posts: 9
Joined: Jan 13, 2005
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I am going with pierce... thank all!
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 Posted Jan 13, 2005, 21:51 PM |
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jay
Group: Member
Posts: 1
Joined: Jan 25, 2005
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pierce
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| Posted Jan 25, 2005, 22:02 PM |
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Spighetta
Group: Member
Posts: 1
Joined: Jan 28, 2005
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Amersham cod. RPN2132
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| Posted Jan 28, 2005, 11:31 AM |
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got777
Group: Member
Posts: 4
Joined: Feb 18, 2005
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biorad immunstar
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| Posted Feb 19, 2005, 0:36 AM |
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bugme
Group: Member
Posts: 9
Joined: Jan 13, 2005
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Yes, Pierce is working good. |
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| Posted Feb 21, 2005, 14:26 PM |
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frasermoss
Group: Admin
Posts: 599
Joined: Feb 22, 2005
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Amersham (GE healthcare as they now are) ECL kits
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"Opportunity is missed by most people because it is dressed in overalls and looks like work". Edison
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| Posted Mar 07, 2005, 0:02 AM |
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RH
Group: Member
Posts: 26
Joined: Mar 16, 2005
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amersham is the best.
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| Posted Mar 21, 2005, 5:13 AM |
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samm
Group: Moderators
Posts: 403
Joined: Mar 03, 2005
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I have found the Pierce SuperSignal West Pico Chemiluminescent kit with stablized peroxide to work very well - my experience with the Pierce product has been better than with Amersham.
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| Posted Mar 21, 2005, 22:51 PM |
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ksettle
Group: Member
Posts: 10
Joined: Mar 31, 2005
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You can also detect mRNA presence/production with a branched-DNA (dBNA) detection probe set for the protein of interest. Results are directly amplified from the mRNA in cell lysate, and yield a chemiluminescent signal that is quantifiable with any light-sensitive reader. (May require a custom probe set if none for your target protein are commercially available).
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| Posted Apr 03, 2005, 15:53 PM |
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nin1318
Group: Member
Posts: 119
Joined: Apr 05, 2005
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| samm said: | | I have found the Pierce SuperSignal West Pico Chemiluminescent kit with stablized peroxide to work very well - my experience with the Pierce product has been better than with Amersham. |
i have used both amersham and pierce...currently use amersham. have had good results with both. |
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| Posted Apr 05, 2005, 22:47 PM |
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badcell
Group: Member
Posts: 13
Joined: Feb 23, 2005
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I too have tried both Pierce and Amersham kits. I don't really like Pierce, though. Amersam ECL and ELC Plus work better in my hands.
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If we knew what it was we were doing, it would not be called research, would it?(A.Einstein)
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| Posted Apr 06, 2005, 19:45 PM |
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camembert
Group: Member
Posts: 31
Joined: Dec 21, 2004
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did pierce satisfy I might use it in the future
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| Posted Apr 12, 2005, 23:06 PM |
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bugme
Group: Member
Posts: 9
Joined: Jan 13, 2005
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| camembert said: | | did pierce satisfy I might use it in the future |
pierce did a very good job. used amersham bioscience nitrocellulose though. |
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| Posted Apr 12, 2005, 16:19 PM |
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MadScientist74
Group: Member
Posts: 15
Joined: Aug 22, 2005
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I use Pierce... but a batch crapped up on me due to production fault... then I tried Santa Cruz... it was good.
Now back to Pierce....
Definitely beats Amersham in terms of stability and luminescence.
My 2 cts.
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| Posted Aug 30, 2005, 3:35 AM |
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