We use non ammoniacal silver staining using reagents as Na2S2O3, AgNO3 + Formaldehyde, developer as Na2CO3 + formaldehyde , stop solution as citrate.

can someone please explain me the principle of using these reagent in silver staining or mechanism behind silver staining.


Please guide me on it earliest.

thanx,

Hi
Sorry for the long waiting
Please find this Article
Electrophoresis. 1990 Oct;11(10):785-94.

Mechanisms of protein silver staining in polyacrylamide gels: a 10-year synthesis.

Rabilloud T.

Biochimie et Physiologie du developpement CNRS URA 686, Ecole Normale Superieure, Paris.

PMID: 1706657 [PubMed - indexed for MEDLINE]

It describes the basics of the different Silver staining methods
Guy