Does anyone know of a good commerical kit for the deglycosylation of proteins under non-denaturing conditions?
From my experience, the only one really working under non-denaturing conditions for moderate to highly glycosylated proteins is the EndoH kit from NEB!
The question sounds ambiguous. I suggest discriminating between enzymatic glycosylation and non-enzymatic glycosylation (glycation) of proteins. Means for deglycosylation in both cases are different.
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