This is a great site for troubleshooting problems with protein electrophoresis. You can learn from the mistakes of others captured on film . . . and you won't believe how bad some of these gels look!

http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html

Thanks for that link.
A very useful one.
I managed to understand things that I saw in the past.

hi there
its good for learners and useful

thnx