Sodium 75Selenite supplier for metabolic labeling |
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Topic Started by jballinger
on 12/14/2006 22:02 PM
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My lab works with a potential selenoprotein, and we would like to confirm this by metabolic labeling in tissue culture using 75Se and subsequent autoradiography of the protein lysate.
Does anyone know where to find a vendor who carries selenium 75 in a bioavailable form such as sodium selenite? I've been searching and haven't had any luck thus far and our Perkin Elmer rep was stumped. Thanks! Any info would be greatly appreciated.
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Replies
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Bioconcentration mechanism of selenium by a coccolithophorid, Emiliania huxleyi. Plant Cell Physiol. 2004 Oct;45(10):1434-41.
http://pcp.oxfordjournals.org/cgi/content/full/45/10/1434#SEC4
These guys obtained [75Se]selenite (H2SeO3 in 0.1 M HCl, 111 MBq ml1, 4,847 GBq g1 from Isotope Products Laboratories, Burbank, CA, U.S.A.
I know that although Amersham used to sell sodium 75Se selenite they have now discontinuted it and don't have a replacement product.
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