HI i am having problem that, my protein is pure 100% and it was purified by isoelctric focusing, when i run this protein in SDS PAGE, I am seeing more bands, i dont know whya, can please explain me. I am obtaining this prure protein from the Iso elctric focusing gels

thanks in advacne

Is that possible that the protein you isolated from IE gel is actually a complex? So when you run it on SDS-PAGE, the complex is sepereated by denaturing condition.

you can try running denaturing gel and native gel side by side and comapre them...