http://www.nottingham.ac.uk/pathology/protocols/solcyanin.html


SECTIONS

8m paraffin wax sections.

SOLUTIONS

1. Solochrome Cyanin Solution

--Solochrome Cyanin RS 0.2g

--Distilled Water 96cm3

--10% Iron Alum 4cm3

--Concentrated Sulphuric Acid 0.5cm3 Add 0.5cm3 concentrated sulphuric acid to 0.2g solochrome cyanin RS in a flask. Stir well until the dye goes into solution. Add 96cm3 distilled water and 4cm3 10% aqueous iron alum. Mix and filter. The solution keeps well.

2. 5% Iron Alum

3. Borax-ferricyanide Differentiator.

--Borax 20g

--Potassium Ferricyanide 25g

--Distilled Water 2000ml
METHOD:

1. Take sections to water

2. Stain in solochrome cyanin solution for 20-30 minutes.

3. Wash in water until the sections turn blue.

4. Differentiate in 5% iron alum 5-10 minutes.

5. Complete the differentiation in borax-ferricyanide differentiator. (The myelin should be blue and the nuclei and background should be cream/white).

6. Dehydrate, clear . Mount sections in DPX


RESULTS:

Myelin and red blood cells - Blue.

Nuclei and background - Colourless