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bacteriophage stx2 phage eg. 933W

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Scot E. DOwd Ph.D.

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Does anyone have a detailed methodology for plaque assay of the stx2 bacteriophage?
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Scot E. Dowd Ph.D.
http://liru.ars.usda.gov
Posted Jan 05, 2005, 19:09 PM
Soudabeh

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Scot E. DOwd Ph.D. said:
Does anyone have a detailed methodology for plaque assay of the stx2 bacteriophage?


Please check the following book:

Sambrook J., and D.W. Russell.2001
Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor. N.Y.

I hope it helps,
Scientist Solutions
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Posted Jan 24, 2005, 22:20 PM
Scot E. DOwd Ph.D.

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Ah thanks! Yeah the basic procedure applies but stx2 phage are a bit more difficult to plaque. We have plaque formation we are looking for methods to acheive larger plaques.
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Scot E. Dowd Ph.D.
http://liru.ars.usda.gov
Posted Jan 24, 2005, 23:51 PM
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