Please find the following protocol:

Small-Scale Selection of Yeast Cells in G1 Phase


Procedure:

1. Grow a lawn of cells in YPD for two days.

2. Wash the cells off the plate with liquid media into a 50 ml tube.

3. Pellet the cells by centrifugation at 1,500 X g for 5 min. Resuspend the cells in 1 M sorbitol in 50% YPD.

4. Centrifuge the cell suspension in a clinical centrifuge at a setting of 4 for 1 min.

5. Recover the cells in the supernatant and discard those in the pellet.

6. Examine the cells under a microscope. If there are still a lot of budded cells, repeat the centrifugation and recover the cells in the supernatant and discard those in the pellet. Examine the cells under a microscope again.


Solutions:

YPD
Autoclave 20 min, cool to room temperature
10 g/liter Yeast Extract
20g/liter D-Glucose (dextrose)
20 g/liter Peptone

50% Sorbitol
50% (w/v) Sorbitol in YPD


Bioreagents and Chemicals:

D-Glucose
Yeast Extract
Sorbitol
Peptone


Reference Link:

http://www.bio.com/protocolstools/protocol.jhtml?id=p819