Here is the procedure I used to anaesthetize the Rats before operation.
A mixture of 50 mg/kg body-weight of Ketamine HCl (Ketaset; Fort Dodge Animal Health, Fort Dodge, Iowa) and 10 mg/kg of Xylazine (Rompun, Bayer Germany) was prepared freshly under sterile conditions.
The mixture was injected interperitonealy.

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