Hi,

I would like to know why "reduced glutathione" is used to elute the protein? why not oxidised?

Tks=)

HI,
To answer your question, first of all GSH - reduced Gluthathaione is the aboundant form in the cell after the cell was exposed to oxidative stress.
So from what I understand there isn't a oxidized gluththione that is stable.
Here is a link to an explanation about GST
http://www.chemicon.com/company/GST.asp

Here is a link for elution of GST
http://www.upstate.com/misc/protocols.q.prot.e.elution/Elution+of+GST+Fusion+Protein+from+Glutathione+Agarose
Guy

THANKS!=)