Anyone use Superblock by Pierce? Do you use it according to Pierce's protocol (fill wells, immediately empty, repeat for a total of 3x)? This uses a lot of reagent.

I used to use SuperBlock for ELISA and Westerns. You can save time by following the wash 3 times directions, but you can also incubate longer (~15-20 minutes) and not do the three washes. Either way works fine.

Lately I've been using the Protein-Free Blocker from Pierce. It seems to have less background in most assays - probably because there is no protein to give nonspecific binding.

Here's the instructions for the Protein-Free Blocker:
http://www.piercenet.com/files/1783as4.pdf