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 How to ligate a fragmet into a vector by the two enzyme digestion truble [View Printable]
whiterabit

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 Send a personal messsage to whiterabit Reply with a quote from this post Go to the top of the page
I want to insert a fragment into the pUC18 by SacI and KpnI, the vicinal restriction enzyme site, but I fail again and again.
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Posted Nov 05, 2006, 8:33 AM
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