mRNA extraction from fixed tissue |
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Topic Started by nin1318
on 11/2/2006 21:49 PM
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has anyone ever used RNA extracted from formaldehyde fixed tissue as a template for real-time?
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Replies
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Posted By Sreenath
on 12/1/2006 10:24 AM
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Yes,
I've once used Formalin fixed tissue for RNA extraction. It is better to incubate the samples at 65 degrees with 50mM NaCl before going for Trizol mediated extraction. I've tried only RT-PCR but never real-time as u had asked for. But I guess it might work in this case too. Let me know if it works.
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Posted By nin1318
on 12/12/2006 17:14 PM
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thanks for the suggestion...what i'm trying to maybe do is do IHC on one half of a brain and qrt-pcr on the other half. i am thinking that it will be difficult...
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