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 immunohistochemistry protocol for human adipose tissue [View Printable]
Rosiglitazone

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I would be so grateful if anyone could anyone tell me where I can find the protocol for immunohistochemistry of human adipose tissue starting at the tissue processing step? Actually there are quite a number of journal articles related to this area, but it seems that none of them provide a detailed discription for this part. Thanks a lot.
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 Posted Oct 26, 2006, 20:44 PM
Zahnklinik

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Hi, That is almost the same procedure as the normal IHC. What you should pa attention is to be very carefulfor each step. what do you want to detect in human adipose tissue?
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Posted Oct 27, 2006, 13:19 PM
gsovak

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Hi
Check this article
jcem.endojournals.org/cgi/reprint/89/10/4833.pdf
It gives a detailed protocol for obtaining the adipose tissue/cells and also IHC.
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Posted Oct 27, 2006, 14:05 PM
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