Hi,
I just discovered this site, it's great that people can offer advice over a forum!

I'm trying to use flow cytometry to sort endothelial cells. Does anyone have a generic protocol? Or better yet, we have the APC-CD31 antibody that I'm using, if anyone has any experience with that can you share it with me please?

Thanks in advance!

CD31 is not endpthelial specific cell marker. I am wondering what the sorting material you are working with? You are working on human endothelial or mouse endothelial? It is hard to help you without knowing your purpose.