Hi, does any one have the experience on angiogenesis assay by endothelial cells? the protocol? Thanks a lot!

I dont personally have experience with the assay, but this article may answer some of your questions.

Angiogenesis Assays: A Critical Overview
Robert Auerbacha1, Rachel Lewis1, Brenda Shinners1, Louis Kubai1 and Nasim Akhtar1

Laboratory of Developmental Biology, University of Wisconsin, 1117 West Johnson St., Madison, WI 53706.

PDF link: http://www.clinchem.org/cgi/reprint/49/1/32

Thank you very much, cfish!