can somebody tell me.how a conserved region can target a multi gene family in gene silencing..and what is the different when i make a construct of hpRNA using 3' end or 5' end...

kema said:

can somebody tell me.how a conserved region can target a multi gene family in gene silencing..and what is the different when i make a construct of hpRNA using 3' end or 5' end...

It sounds to me like you want to find a region of the gene that is conserved in all memebers of the gene family, and use that as a target site for your siRNA. So the first step would be to do a multiple sequence alignment of all of the mRNA sequences to see if such a highly conserved region exists.