Does anybody know whether human angiogenin is inside the cell or on the cell surface? Do all kind of human cells have it? Its concentration inside the cell is low or kind of high? Thanks a lot.

Fogee - as far as I understand it, angiogenin is a circulating blood protein in the RNAse superfamily and it may be secreted from the pancreas although I am not sure about that. So it is neither inside the cell nor on the cell surface. I will check at www.rcsb.org to see if the X-ray crystal structure has been determined. Those serum proteins are usually pretty robust and some of the easiest to purify and crystallize

Thanks for your information. It actually has crystal structure. It seems to stay in serum most of time, but can travel into the cell sometimes since it can have nucleus translocation.

Does anybody know how I can find out the rough concentration it has inside some specific cells? Or the chemicals which can induce more angiogenin?

If something which can upregulate its mRNA, does it mean its protein expression will be higher? Thanks.