Dear Forum members,
I am working on the erythromycin producing by Saccharopolyspora erythraea which is from USA, now have some questions. Production media is soybean powder 2.0%,yeast powder 0.5%,(NH4)2SO4 0.032%, bean oil 6.0%,CaCO3 0.75%, CuCl22H2O 0.0035%. The mycelium will autolyze after 120 hour during fermentation.
I am waiting for your response,
Thanks in advances

yao:

Can you describe your question in more detail? Are you concerned with controlling the autolyze characteristics of your fermentation culture?

trook :

Yes, The autolyze is not what I want.
The natural cycle of fermentiation should be 180 hour,but it often autolyze in 120 hour, and the yield is only 6.0-6.5g/L.
I want to increase erythromycin productibity to 8.5-9.0g/L.But I don't know what is wrong with it.

Any help would be appreciated

yao:

I'm not sure what organism you are using but the following review article gives quite a few suggestions about maintaining a stable transformant for biotech fermentation manufacturing.

Schallmey M, Singh A, Ward OP. Developments in the use of Bacillus species for industrial production. Can J Microbiol 2004, 50: 1-17.

Good luck! And please let the board know how things went.

thank you.