metagenomics [View Printable]
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fenpatel
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Posts: 4
Joined: Sep 22, 2006
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WHAY AREW THE STEPS TO ISOLATE MICROBIAL DNA FROM SOIL AND ENVIROAMENT |
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| Posted Sep 22, 2006, 16:58 PM |
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ryan_m
Group: Moderators
Posts: 280
Joined: May 06, 2006
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| fenpatel said: | | WHAT ARE THE STEPS TO ISOLATE MICROBIAL DNA FROM SOIL AND ENVIRONMENT |
I am assuming you are interested in extracting DNA from soil to do an environmental sampling (sequencing) of a given soil sample. There are many good reviews on the current methods of preparing these samples. Here is where I would start looking: J Microbiol Methods. 2005 Oct;63(1):1-19. J Microbiol Methods. 2004 Aug;58(2):169-88. Appl Environ Microbiol. 2005 Oct;71(10):5710-8. |
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| Posted Sep 22, 2006, 17:10 PM |
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fenpatel
Group: Member
Posts: 4
Joined: Sep 22, 2006
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IS ANY POSSIBILITY THAT FROM MEGAGENOM PARTICULAR BACTERIA CAN IDENTIFID? |
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| Posted Sep 22, 2006, 18:08 PM |
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ryan_m
Group: Moderators
Posts: 280
Joined: May 06, 2006
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| fenpatel said: | | IS ANY POSSIBILITY THAT FROM MEGAGENOM PARTICULAR BACTERIA CAN IDENTIFID? |
The possibility exists, yes. Keep in mind that one of the best ways to identify a given bacterium is to sequence its ribosomal RNA genes. If your goal is to identify the bacteria present in a sample, a more affordable approach may be to use primers to amplify these genes from your sample and clone/sequence the fragments. You should be able to use these sequences to create a catalog of some of the more prevalent bacteria in the sample. |
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| Posted Sep 22, 2006, 18:22 PM |
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fenpatel
Group: Member
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Joined: Sep 22, 2006
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AFTER ISOLATION OF PARTICULAR DNA SEGMENT, CAN WE CULTURE THE BACTERIA ON THE BASIS OF DNA EXPRESSION? |
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| Posted Sep 23, 2006, 18:56 PM |
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ryan_m
Group: Moderators
Posts: 280
Joined: May 06, 2006
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I'm not sure I understand you. Do you mean that identifying a particular bacterium (using rRNA genes, let's say) will allow you to isolate (e.g. culture) that bacterium? I'm not sure it would be possible using only your knowledge that the bacterium is there. |
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| Posted Sep 23, 2006, 19:19 PM |
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fenpatel
Group: Member
Posts: 4
Joined: Sep 22, 2006
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WHEN WE INSERT PARTICULAR GENE IN MODEL MICROORGANISM. SO, BY ANALYSING ITS EXPRESSION I THINK WE CAN FIND OUT WHICH SUBSTRATE IT USE, BY COMPRING WITH OTHER MICROORGANISM OF SAME EXPRESSION I THINK WE CAN FIND OUT WHICH SUBSTRATE IT USE |
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| Posted Sep 24, 2006, 5:36 AM |
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