Hello,

Has anyone worked with transplatin in cell culture tox assays and found it to be toxic ?
I'm working with the drug (300 micromolar) using short incubation times (2 hours) in ovarian cancer cells. I'm getting reproducible IC50 values 24 h later of around 150 micromolar.
The drug is dissolved in EBSS by vortexing and brief sonication using a probe sonicator on low setting.

Many thanks for any thoughts

Though I dont know your area very much. 300uM of any drug treatment looks to me quite a high in concentration

Yeah, it's a short contact time and it's standard practice for this FDA/EMEA approved protocol that I'm using (Top conc = 100mg/ml, which is about 300 micromolar drug).

Hi Delilah, check out this paper:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8396390&dopt=Citation

Thanks for that info.