Scientist Solutions: International Life Science Community By Scientists For Scientists
    
Home » Forums » Analytical Chemistry » Assay Development and Protocols » 2d chromatography

Thanks to our sponsors who make this site possible
Search forums by keyword   or   Create a new topic
 Link To Us    Bookmark Us     Chinese

2d chromatography

 RSS Feed

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Close Window 
View Printable Version
humaira
India

Send PM
See Mini bio

Status: Frog Egg
Frog Egg
Topic Started by humaira
on 8/16/2006 17:25 PM   
Reply to this post Go to the top of the page


please can any body help me out for the procedure of 2d chromatography of amino acids
Replies
cfish
United States

Send PM
See Mini bio

Status: Animal & Vet Science, Biochemistry & Biophysics Moderator
Frog Laureate
Posted By cfish
on 2/15/2007 17:39 PM   
Reply to this post Go to the top of the page

2 Dimensional Thin Layer Cellulose Electrophoresis of Phosphoamino Acids

Overview

This protocol describes the electrophoretic separation of phosphorylated amino acids on Thin Layer Cellulose. This procedure often follows metabolic labeling of cellular proteins by 35S or 32P and tryptic peptide digestion of polypeptides (see Protocol ID#310). This procedure requires a horizontal flatbed platform, with electrodes embedded in reservoirs at the ends of the platform, and cooling tubes for running cold water underneath the flatbed. The phosphorylated amino acids can also be analyzed by High Pressure Liquid Chromatography (see Protocol ID#591).

Link: http://www.bio.com/protocolstools/protocol.jhtml?id=p2155


Phosphoamino Acid Analysis
Link: http://www.bio.com/protocolstools/protocol.jhtml?id=p312
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.

By participating in the voting, you will be helping to identify the best topics & replies on the board.

You may vote once for any one post, and you may not vote for your own posts.

A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.

learn more about member points.

Close Window 


Get our
RSS feed
Follow us
on Twitter
Refer
a colleague
Click here to
Become a member & join our
community (It's easy & free)
Already a member? Please log in
User Name  
Password  
Forget Password?
Find out about our Rewards System
Scientists
Not finding the answer you need?

 Post a new topic

You must be logged in to post. Log in above.
Not a member yet? Click here to register
(it's free)
Thank You to Our Sponsor

The Latest Events

Board Rules | advertise | Privacy

Copyright © 2004-2011 Scientist Solutions, All Rights Reserved.
Resources from NCBI used on this site.