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post translation of modifcation [View Printable]
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Mk
Group: Member
Posts: 1
Joined: Jul 28, 2006
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Hi every body
Can any body help me for my protien modfiacation, I myc tag my protein and did westran , I saw three bands of my protein , two very close and one 15 kd down. This happen to my protein when my yeast cell undergo meiosis. its strats from time point 4 in yeast meiotic time course. i don,t know what type of modifacation is it?
can any body help?
Cheers
Mk
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 Posted Jul 28, 2006, 21:28 PM |
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frasermoss
Group: Moderators
Posts: 527
Joined: Feb 22, 2005
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You could try treating your cultures with N-glycosydase F to look at glycosylation This drug cleaves asapargine linked glycan chains on glycoproteins. You can purchase a "N-glycosidase F deglycosylation kit" from Roche. Here is the brochure http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.4_96/p13.pdf |
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"Opportunity is missed by most people because it is dressed in overalls and looks like work". Edison
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| Posted Jul 29, 2006, 16:53 PM |
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R Bishop
Group: Moderators
Posts: 243
Joined: Jan 17, 2006
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I agree with Frasermoss. Yeast are very good at putting glycosylation on proteins. Check your protein sequence for Asn linkage sites for N-glycosylation (Asn-Xaa-Ser/Thr) before you buy the digest kit though.
Rb
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| Posted Jul 31, 2006, 22:23 PM |
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