Hi all,
I would like to do a 2D for a membrane channel protein.
Any one has an experience with this?

2D Crystallization of Membrane Proteins: Rationales and Examples

http://walz.med.harvard.edu/Publications/PDFs/Hasler-JSB-1998.pdf

it's it old now but it's a start

You may also want to look up Rod Mackinnon and his work - Nobel prize winner for crystalizing potassium channels and other membrane proteins

http://www.rockefeller.edu/labheads/mackinnon/mackinn-lab.php