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Quantifying Chemiluminescence using image J

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montgomm

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Which plug-in or what function of image J do people use to quantify their bands from in western blots?

I've been using the "Analyze/Gel" function, but not sure that is actually the correct way to go.

Suggestions?

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Posted Jul 19, 2006, 4:57 AM
lscraig

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you use a mac or pc?

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Posted Sep 28, 2006, 22:43 PM
montgomm

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I use PC

I found out the best way is to use the Ctrl +M "Measure function" for each band and in the measurement options select "Integrated Density"

This has worked very well since I implemented it.

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Posted Sep 29, 2006, 22:55 PM
gsovak

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Any one got an old version for an old PC.
The one that I downloaded from the site needs a Mac OSX I got Mac OS IX
Guy

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Posted Feb 01, 2007, 23:30 PM
vanishing

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you could also consider this alternatives

Gelscape
http://www.gelscape.ualberta.ca:8080/htm/index.html

(no experience with that I must admit)

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Posted Feb 06, 2007, 3:37 AM
qinglongyanyuedao

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for the Ctrl + M, you will need first do the color invert

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Posted Apr 02, 2007, 15:51 PM
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